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Comparison of two prevalent individually ventilated caging systems for detection of murine infectious agents via exhaust air particles

Author:
Mahabir, Esther, Durand, Stephanie, Henderson, Kenneth S, Hardy, Patrick
Source:
Laboratory animals 2019 v.53 no.1 pp. 84-88
ISSN:
1758-1117
Subject:
Entamoeba, Helicobacter, Pasteurella, air, cages, laboratory animals, mice, monitoring, pathogens, quantitative polymerase chain reaction
Abstract:
We compared the efficacies of two different individually ventilated cage systems (Allentown and Tecniplast) for health monitoring (HM) of murine infectious agents using exhaust air particle (EAP) capture and real-time PCR. After three months of monitoring, both EAP capture media allowed detection of Helicobacter, Pasteurella and Entamoeba. Use of the EAP real-time PCR for HM reduces the number of mice used.
Agid:
6459265