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Mucilaginibacter endophyticus sp. nov., an endophytic polysaccharide-producing bacterium isolated from a stem of Miscanthus sinensis

Zhang, Zhendong, Sun, Feng, Chen, Yan, Yao, Lunguang, Chen, Zhaojin, Tian, Wei
Antonie van Leeuwenhoek 2019 v.112 no.7 pp. 1087-1094
Miscanthus sinensis, Mucilaginibacter, bacteria, endophytes, exopolysaccharides, fatty acid composition, fatty acids, genome, genomics, isoprenoids, nucleotide sequences, phylogeny, quinones, ribosomal RNA
In this study, a Gram-negative, rod-shaped, endophytic bacterial strain (RS1ᵀ) capable of producing large amounts of exopolysaccharides was isolated from a stem of Miscanthus sinensis. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain RS1ᵀ is closely related to Mucilaginibacter kameinonensis NBRC 102645ᵀ (98.72%), followed by Mucilaginibacter gossypiicola Gh-48ᵀ (97.56%) and Mucilaginibacter oryzae DSM 19975ᵀ (97.36%). The DNA G + C content of strain RS1ᵀ was determined to be 42.80 mol%. ANIb and GGDC values from genomic comparison between the genomes of strain RS1ᵀ and the related reference species were less than 95% and 70%, respectively. The major cellular fatty acids (more than 10% of total fatty acids) were identified as iso-C15: 0, C16:0, iso-C17:0-3OH and summed feature 3 (C16: 1ω7c and/or iso-C15:02-OH). The only isoprenoid quinone detected was MK-7. Based on the physiological, genotypic and genomic characteristics, strain RS1ᵀ is concluded to represent a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter endophyticus sp. nov. is proposed (type strain RS1ᵀ = KCTC 62785ᵀ = GDMCC 1.1414ᵀ).