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In Vivo Near-Infrared Fluorescence and Photoacoustic Dual-Modal Imaging of Endogenous Alkaline Phosphatase

Author:
Gao, Xiaoting, Ma, Gongcheng, Jiang, Chao, Zeng, Leli, Jiang, Shanshan, Huang, Peng, Lin, Jing
Source:
Analytical chemistry 2019 v.91 no.11 pp. 7112-7117
ISSN:
1520-6882
Subject:
alkaline phosphatase, biomarkers, dyes, fluorescence, image analysis, moieties, neoplasms, phosphates, tissues
Abstract:
Alkaline phosphatase (ALP) is distributed widely in living organisms and is an important biomarker closely related to many physiological and pathological processes. However, in vivo real-time detection of ALP remains a significant challenge. Herein, we developed a turn-on molecular probe (denoted as LET-3) to visualize ALP activity in tumor tissues through near-infrared fluorescence (NIRF) and photoacoustic (PA) dual-modal imaging. LET-3, composed of NIR hemicyanine dye (LET-CyOH) and a phosphate moiety, showed a 23-fold NIRF enhancement at 730 nm and 27-fold PA enhancement at 710 nm upon activation by ALP. More importantly, both in vitro and in vivo diagnostic experiments indicated that LET-3 has a high sensitivity and good selectivity for ALP. These findings provide a promising strategy for in vivo ALP detection using NIRF and PA dual-channel turn-on probes.
Agid:
6459556