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Novel Soy β-Conglycinin Core–Shell Nanoparticles As Outstanding Ecofriendly Nanocarriers for Curcumin

Author:
Liu, Ling-Ling, Liu, Peng-Zhan, Li, Xiu-Ting, Zhang, Ning, Tang, Chuan-He
Source:
Journal of agricultural and food chemistry 2019 v.67 no.22 pp. 6292-6301
ISSN:
1520-5118
Subject:
beta-conglycinin, bioactive compounds, bioavailability, crystals, curcumin, dialysis, dietary supplements, drugs, encapsulation, functional foods, globulins, hydrophobicity, nanocarriers, nanoparticles, thermal stability, urea, water solubility
Abstract:
The development of high-performance nanocarriers for nutraceuticals or drugs has become one of the topical research subjects in the functional food fields. In this work, we for the first time propose a novel and ecofriendly process to obtain a kind of nanostructured soy β-conglycinin (β-CG; a major soy storage globulin) as outstanding nanocarriers for poorly soluble bioactives (e.g., curcumin), by a urea-assisted disassembly and reassembly strategy. At urea concentrations > 4 M, the structure of β-CG gradually dissociated into its separate subunits (α, α′, and β) and even denatured (depending on the type of subunits); after dialysis to remove urea, the dissociated subunits would reassemble into a kind of core–shell nanostructured particles, in which aggregated β-subunits acted as the core while the shell layer was mainly composed of α- and α′-subunits. The core–shell nanoparticles were favorably formed at protein concentrations of 1.0–2.0 wt %. Curcumin crystals were directly introduced into the β-CG solution at high urea concentrations (e.g., 8 M) and would preferentially interact with the denatured β-subunits. As a consequence, almost all of the curcumin molecules were encapsulated in the core part of the reassembled core–shell nanoparticles. The loading amount of curcumin in these nanoparticles could reach 18 g of curcumin per 100 g of protein, which far exceeds those reported previously. The encapsulated curcumin exhibited a high water solubility, extraordinary thermal stability, and improved bioaccessibility, as well as a sustained release behavior. The findings provide a novel strategy to fabricate a kind of high-encapsulation-performance, organic solvent-free, and biocompatible nanocarrier for hydrophobic nutraceuticals and drugs.
Agid:
6459868