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Quantitative Proteomics Analysis of Barley-Based Liquid Feed and the Effect of Protease Inhibitors and NADPH-Dependent Thioredoxin Reductase/Thioredoxin (NTR/Trx) System
- Sultan, Abida, Andersen, Birgit, Christensen, Jesper Bjerg, Poulsen, Hanne Damgaard, Svensson, Birte, Finnie, Christine
- Journal of agricultural and food chemistry 2019 v.67 no.22 pp. 6432-6444
- digestibility, feed additives, feeding methods, grains, liquids, livestock, monitoring, nutrient availability, outer membrane proteins, oxidoreductases, proteinase inhibitors, proteolysis, proteomics, seed storage, soaking, solubility, storage proteins, thioredoxins
- Liquid feeding strategies have been devised with the aim of enhancing grain nutrient availability for livestock. It is characterized by a steeping/soaking period that softens the grains and initiates mobilization of seed storage reserves. The present study uses 2D gel-based proteomics to investigate the role of proteolysis and reduction by thioredoxins over a 48 h steeping period by monitoring protein abundance dynamics in barley-based liquid feed samples supplemented with either protease inhibitors or NADPH-dependent thioredoxin reductase/thioredoxin (NTR/Trx). Several full-length storage proteins were only identified in the water-extractable fraction of feed containing protease inhibitors, illustrating significant inhibition of proteolytic activities arising during the steeping period. Application of functional NTR/Trx to liquid feed reductively increased the solubility of known and potentially new Trx-target proteins, e.g., outer membrane protein X, and their susceptibility to proteolysis. Thus, the NTR/Trx system exhibits important potential as a feed additive to enhance nutrient digestibility in monogastric animals.