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Synthesis, Optimization, and Evaluation of Glycosylated Naphthalimide Derivatives as Efficient and Selective Insect β-N-Acetylhexosaminidase OfHex1 Inhibitors

Shen, Shengqiang, Dong, Lili, Chen, Wei, Wu, Renjie, Lu, Huizhe, Yang, Qing, Zhang, Jianjun
Journal of agricultural and food chemistry 2019 v.67 no.22 pp. 6387-6396
Myzus persicae, Ostrinia furnacalis, Plutella xylostella, beta-N-acetylhexosaminidase, bioactive properties, computer simulation, glycosylation, insects, molecular dynamics, pest control, pesticides, pests, simulation models
Insect chitinolytic β-N-acetylhexosaminidase OfHex1, from the agricultural pest Ostrinia furnacalis (Guenée), is considered as a potential target for green pesticide design. In this study, rational molecular design and optimization led to the synthesis of compounds 15r (Kᵢ = 5.3 μM) and 15y (Kᵢ = 2.7 μM) that had superior activity against OfHex1 than previously reported lead compounds. Both compounds 15r and 15y had high selectivity toward OfHex1 over human β-N-acetylhexosaminidase B (HsHexB) and human O-GlcNAcase (hOGA). In addition, to investigate the basis for the potency of glycosylated naphthalimides against OfHex1, molecular docking and molecular dynamics simulations were performed to study possible binding modes. Furthermore, the in vivo biological activity of target compounds with efficient OfHex1 inhibitory potency was assayed against Myzus persicae, Plutella xylostella, and O. furnacalis. This present work indicates that glycosylated naphthalimides can be further developed as potential pest control and management agents targeting OfHex1.