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A designer natural deep eutectic solvent to recycle the cofactor in alcohol dehydrogenase-catalysed processes
- Mourelle-Insua, Ángela, Lavandera, Iván, Gotor-Fernández, Vicente
- Green chemistry 2019 v.21 no.11 pp. 2946-2951
- alcohol dehydrogenase, biotransformation, choline, glucose, green chemistry, ketones, nicotinamide, solvents
- Deep eutectic solvents (DESs) nowadays represent a sustainable alternative to traditional organic solvents in (bio)transformations. Herein, the use of a solvent composed of an aqueous buffer and choline chloride : glucose (1.5 : 1 mol/mol) is proposed, a natural DES (NADES) serving as both a cosolvent and efficient system to recycle the nicotinamide cofactor. Thus, glucose from the NADES served as a co-substrate required for several alcohol dehydrogenases to reduce different prochiral ketones, and also helped to solubilise the organic compounds to develop effective biotransformations at higher substrate concentrations.