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Circular RNA circ_0020123 promotes non-small cell lung cancer progression by acting as a ceRNA for miR-488–3p to regulate ADAM9 expression

Author:
Wan, Jingru, Hao, Liguo, Zheng, Xiaoyang, Li, Zheng
Source:
Biochemical and biophysical research communications 2019 v.515 no.2 pp. 303-309
ISSN:
0006-291X
Subject:
apoptosis, bioinformatics, cell lines, cell viability, circular RNA, databases, flow cytometry, high-throughput nucleotide sequencing, luciferase, lung neoplasms, lymph nodes, metastasis, neoplasm progression, non-coding RNA, patients, prediction, prognosis, quantitative polymerase chain reaction, reporter genes, reverse transcriptase polymerase chain reaction, therapeutics, tissues
Abstract:
Circular RNAs (circRNAs) is a class of non-coding RNA with important functions in tumor development and progression. In the previous study, circ_0020123 was found to be an elevated circRNA in non-small cell lung cancer (NSCLC) tissues screened by high-throughput sequencing. In the current work, we uncovered the clinical significance, biological functions and mechanism of circ_0020123 in NSCLC. The expression profile of circ_0020123 was measured by RT-qPCR. Correlations between circ_0020123 expression and patients’ clinical features were evaluated. Cell viability, apoptosis, migration and invasion were detected by cell counting kit-8 (CCK-8), flow cytometric and transwell assay, respectively. Bioinformatics database and luciferase reporter gene assays were utilized to identify the mechanisms of circ_0020123. The results revealed elevation of circ_0020123 in tissue specimens and cells than the nontumorous tissues and 16HBE, separately. The enhancement of circ_0020123 in tumor tissues correlated with positive lymph node metastasis, advanced TNM stages, and adverse prognosis for NSCLC patients. Functionally, silencing of circ_0020123 distinctly suppressed the growth, migration and invasion and inhibited the apoptosis of A549 cells. On the contrary, when circ_0020123 expression was ectopically expressed, the above effects were significantly strengthened in H1299 cell line. For the mechanism exploration, circ_0020123 could sponge miR-488–3p to release its inhibition on ADAM9 expression. Moreover, the functional role of circ_0020123 is partly dependent on its regulation of ADAM9 proved by rescue assays. Taken together, our findings provide the possibility that circ_0020123 may be a new target for NSCLC prognosis prediction and therapy.
Agid:
6460510