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Continuous dynamics in anaerobic reactor during bioconversion of rice straw: Rate of substance utilization, biomethane production and changes in microbial community structure

Wachemo, Akiber Chufo, Tong, Huan, Yuan, Hairong, Zuo, Xiaoyu, Korai, Rashid Mustafa, Li, Xiujin
The Science of the total environment 2019 v.687 pp. 1274-1284
Enterobacteriaceae, Methanobacterium, Methanosaeta, Methanosarcina, Peptostreptococcaceae, acetic acid, anaerobic digesters, anaerobic digestion, biogas, biotransformation, carbon dioxide, community structure, gas production (biological), methane production, microbial communities, propionic acid, rice straw
Anaerobic digestion (AD) technology is one of the best eco-friendly waste-to-energy processes. Hence the trend of biogas production shows considerable ranges of fluctuations in entire digestion period. This study analyzes the daily substance conversion dynamics, biomethane production and changes in microbial community structure. The results show that in the first peak of biogas production during 1st up to 4th days of the digestion period, CO2 was the dominant component. However, the daily methane production (DMP) reveal accelerated increment starting from day 8 up to the peak point on day 13 (462.11 mL/d). The concentration of acetic acid covered 21.80% up to 62.00% of the total VFAs in the first 10 days of digestion period. On the other hand, the accumulation of propionic acid is in the range of 1735.70 mg/L- 2893.12 mg/L in between day 5–15, which is beyond the inhibition level (1000 mg/L) but the system didn't stop biogas production. The distribution of bacterial family such as Clostridiaceae_1, Prevotellaceae, Enterobacteriaceae and Peptostreptococcaceae are the dominant group at early stage as compared to composition in remaining stages except Enterobacteriaceae which have marginally high abundance in lowest biogas production point. The archaeal genus Methanosaeta is dominant among the samples collected at early stages (65.66%–77.22%). However, the Methanobacterium is predominant (34.88%–59.40%) in samples obtained at late stages of AD period. On the other hand, the distribution of Methanosarcina is comparable in the first three samples (S1(16.60%), S2(22.21%) and S3(20.38%)) than the stable stage (S4 (6.7%)). This study demonstrates the detailed conditions at fluctuating and constant biogas production periods, which would benefit future researchers working in similar area.