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Molecular and functional characterization of odorant-binding protein genes in Holotrichia oblita Faldermann
- Wang, Chao-Qun, Li, Jin-Qiao, Li, Er-Tao, Nyamwasa, Innocent, Li, Ke-Bin, Zhang, Shuai, Peng, Yu, Wei, Zhao-Jun, Yin, Jiao
- International journal of biological macromolecules 2019 v.136 pp. 359-367
- Coleoptera, RNA interference, antennae, attractants, complementary DNA, double-stranded RNA, fluorescence, genes, messenger RNA, molecular cloning, nucleotide sequences, odorant receptors, p-cresol, pests, sequence analysis, skatole
- Holotrichia oblita is one of the nastiest pests in China. In present research, four full-length cDNA encoding of HoblOBP genes were cloned and sequenced from H. oblita. The mRNA of HoblOBPs were predominantly expressed in antenna. The recombinant HoblOBPs proteins were obtained for fluorescence binding assays. Four of HoblOBPs could mediate the response of H. oblita to organic fertilizers-derived attractants, including HoblOBP5 binding to skatole; HoblOBP8 binding to p-cresol, indole and skatole; HoblOBP9 binding to indole and 4-allylanisole; and HoblOBP24 binding to p-cresol, indole and 4-ethylphenol. Further, RNA interference demonstrated that transcripts of HoblOBP5, 8, 9, and 24 decreased in a time-dependent manner after dsRNA-injection. Knockdown of HoblOBP5, 8, 9, and 24 by injection of dsRNA successfully interfered with behavioral responses towards the target compounds in beetles. Our results showed that HoblOBP5, HoblOBP8, HoblOBP9 and HoblOBP24 are essential in mediating the approach behavior of H. oblita.