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Enhancement of lipid accumulation in Monoraphidium sp. QLY-1 by induction of strigolactone

Author:
Song, Xueting, Zhao, Yongteng, Li, Tao, Han, Benyong, Zhao, Peng, Xu, Jun-Wei, Yu, Xuya
Source:
Bioresource technology 2019 v.288 pp. 121607
ISSN:
0960-8524
Subject:
Monoraphidium, biomass, biosynthesis, calcium, cell growth, fuel production, gene expression, gene expression regulation, lipid content, lipids, microalgae, nitric oxide, strigolactones, transcription (genetics)
Abstract:
In this study, the effects of strigolactone (SL) on the biomass, lipid content, biochemical properties, and gene transcription of Monoraphidium sp. QLY-1 were examined. The lipid content and lipid productivity increased by 61% and 55% in QLY-1 under 1 μM SL induction compared to the control group, respectively. SL also upregulated the levels of endogenous NO and Ca2+ and lipid biosynthesis gene transcription. Subsequently, the relationship between Ca2+ and nitric oxide (NO) in the regulation of cell growth and lipid accumulation of QLY-1 under SL induction conditions was analysed. An increase in endogenous Ca2+ regulated cell growth and lipid biosynthesis by modulating the levels of NO and lipid biosynthesis-related gene expression. Collectively, this study provided a valuable approach for biofuel production from microalgae under SL induction and demonstrated that there is crucial crosstalk between the Ca2+ and NO signalling in the manipulation of lipid biosynthesis in microalgae under SL treatment.
Agid:
6462416