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Enhancement of lipid accumulation in Monoraphidium sp. QLY-1 by induction of strigolactone

Song, Xueting, Zhao, Yongteng, Li, Tao, Han, Benyong, Zhao, Peng, Xu, Jun-Wei, Yu, Xuya
Bioresource technology 2019 v.288 pp. 121607
Monoraphidium, biomass, biosynthesis, calcium, cell growth, fuel production, gene expression, gene expression regulation, lipid content, lipids, microalgae, nitric oxide, strigolactones, transcription (genetics)
In this study, the effects of strigolactone (SL) on the biomass, lipid content, biochemical properties, and gene transcription of Monoraphidium sp. QLY-1 were examined. The lipid content and lipid productivity increased by 61% and 55% in QLY-1 under 1 μM SL induction compared to the control group, respectively. SL also upregulated the levels of endogenous NO and Ca2+ and lipid biosynthesis gene transcription. Subsequently, the relationship between Ca2+ and nitric oxide (NO) in the regulation of cell growth and lipid accumulation of QLY-1 under SL induction conditions was analysed. An increase in endogenous Ca2+ regulated cell growth and lipid biosynthesis by modulating the levels of NO and lipid biosynthesis-related gene expression. Collectively, this study provided a valuable approach for biofuel production from microalgae under SL induction and demonstrated that there is crucial crosstalk between the Ca2+ and NO signalling in the manipulation of lipid biosynthesis in microalgae under SL treatment.