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PnSAG1, an E3 ubiquitin ligase of the Antarctic moss Pohlia nutans, enhanced sensitivity to salt stress and ABA

Wang, Jing, Liu, Shenghao, Liu, Hongwei, Chen, Kaoshan, Zhang, Pengying
Plant physiology and biochemistry 2019 v.141 pp. 343-352
Arabidopsis thaliana, Physcomitrella patens, abscisic acid, cold, cytoplasm, drought, enzyme activity, gametophytes, gene expression regulation, genes, mosses and liverworts, plant response, quantitative polymerase chain reaction, reverse transcriptase polymerase chain reaction, root growth, salinity, salt stress, salt tolerance, seed germination, stress response, ubiquitin, ubiquitin-protein ligase, ubiquitination, Antarctic region
Plant U-box (PUB) E3 ubiquitin ligases play crucial roles in the plant response to abiotic stress and the phytohormone abscisic acid (ABA) signaling, but little is known about them in bryophytes. Here, a representative U-box armadillo repeat (PUB-ARM) ubiquitin E3 ligase from Antarctic moss Pohlia nutans (PnSAG1), was explored for its role in abiotic stress response in Arabidopsis thaliana and Physcomitrella patens. The expression of PnSAG1 was rapidly induced by exogenous abscisic acid (ABA), salt, cold and drought stresses. PnSAG1 was localized to the cytoplasm and showed E3 ubiquitin ligase activity by in vitro ubiquitination assay. The PnSAG1-overexpressing Arabidopsis enhanced the sensitivity with respect to ABA and salt stress during seed germination and early root growth. Similarly, heterogeneous overexpression of PnSAG1 in P. patens was more sensitive to the salinity and ABA in their gametophyte growth. The analysis by RT-qPCR revealed that the expression of salt stress/ABA-related genes were downregulated in PnSAG1-overexpressing plants after salt treatment. Taken together, our results indicated that PnSAG1 plays a negative role in plant response to ABA and salt stress.