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Comparative impact of coumaphos, amitraz and plant extract of Ageratum conyzoides on the oogenesis of Rhipicephalus microplus

Author:
K.G., Ajith Kumar, Fular, Ashutosh, Chigure, Gajanan, Sharma, Anil Kumar, Nagar, Gaurav, Souza, Francinea F., Bechara, Gervasio H., Ghosh, Srikant
Source:
Ticks and tick-borne diseases 2019 v.10 no.5 pp. 1085-1095
ISSN:
1877-959X
Subject:
Ageratum conyzoides, Rhipicephalus microplus, adults, amitraz, chorion, color, coumaphos, deformation, egg yolk, eggs, females, lipids, oocytes, oviducts, oviposition, plant extracts, polysaccharides, proteins, tick-borne diseases, ticks, uterus, vitellogenesis
Abstract:
The present experiment was conducted to evaluate and compare the impact of Ageratum conyzoides plant extract (ACE) with routinely used synthetic acaricides i.e., amitraz and coumaphos on the oogenesis of engorged adult females of Rhipicephalus microplus tick. On the day of dropping from the host, panoistic ovary of R. microplus appeared white in colour, horseshoe shaped, hollow tubular organ with immature oocytes predominantly in dorsal groove. Different developmental stages of oocytes (I–V) proceed simultaneously and asynchronously. Oocytes showed gradual increase in size, deep brown colored with accumulation of eggs in oviduct during 24–72 hours of development.At LC90 concentration a highly significant (p < 0.001) cessation of egg laying after exposure to amitraz and ACE while significant reduction (p < 0.01) of egg laying in coumaphos treated ticks was observed. Upon dissection of treated ticks, uterus and oviduct packed with eggs, which failed to pass out was observed. The histo-architectural alterations including presence of extensive vacuolation, alteration of oocyte morphology, deformation of chorion and disorganization of yolk granules were observed in the treated ovaries. Histochemically, low level of storage or synthesis of essential elements viz., proteins, polysaccharides and lipids in treated oocytes responsible for reduction of fertility and inhibition of progress of vitellogenesis was observed.
Agid:
6462709