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First description of Giardia duodenalis in buffalo calves (Bubalus bubalis) in southwest region of São Paulo State, Brazil

de Aquino, Monally Conceição Costa, Harvey, Tatiani Vitor, Inácio, Sandra Valéria, Nagata, Walter Bertequini, Ferrari, Elis Domingos, Oliveira, Bruno César Miranda, Albuquerque, George Rego, Widmer, Giovanni, Meireles, Marcelo Vasconcelos, Bresciani, Katia Denise Saraiva
Food and Waterborne Parasitology 2019 v.16 pp. e00062
Bubalus bubalis, Giardia lamblia, buffaloes, calves, feces, genes, genetic similarity, giardin protein, glutamate dehydrogenase, polymerase chain reaction, ribosomal RNA, sequence analysis, triose-phosphate isomerase, Brazil
We performed molecular characterization of Giardia duodenalis in buffalo calves from the Southwest region of São Paulo State, Brazil. A total of 183 fecal samples of Murrah breed buffaloes up to six months of age were collected. We examined these samples by the polymerase chain reaction (PCR) targeting the small-subunit ribosomal RNA gene and positive samples were characterized using additional PCR assays targeting a portion of the beta-giardin, the glutamate dehydrogenase and the triose-phosphate isomerase genes. Based on the SSU rRNA nPCR, the presence of G. duodenalis was confirmed in 12 (6.56%) of fecal samples, of these, five, four and three samples were positive for the tpi, bg and gdh genes, respectively. Assemblage identification by sequencing was successful in 6 of 12 samples and sequence analysis showed 100% genetic similarity with G. duodenalis assemblage E. This observation represents the first detection of G. duodenalis assemblage E in buffaloes calves in Brazil.