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Pyruvate Responsiveness Based on α-Oxohydrazone Formation for Intracellular siRNA Release from Polyion Complex-Based Carriers

Author:
Takemoto, Hiroyasu, Wang, Chih-Ling, Nomoto, Takahiro, Matsui, Makoto, Tomoda, Keishiro, Nishiyama, Nobuhiro
Source:
Biomacromolecules 2019 v.20 no.6 pp. 2305-2314
ISSN:
1526-4602
Subject:
cytoplasm, electrostatic interactions, gene silencing, hydrazides, moieties, polymers, pyruvic acid, small interfering RNA
Abstract:
Selective release of small interfering RNA (siRNA) payloads in response to intracellular substances is a prerequisite for the smart design of siRNA carriers. In this context, we developed a molecular program that allows reactivity with pyruvate for siRNA release in the cell on the basis of polyionic-complex- (PIC-) based siRNA carriers. Hydrazide can react with the α-keto acid structure of anionic pyruvate to form α-oxohydrazone, resulting in the reduction of the cationic net charge of the cationic polymer bearing a hydrazide moiety, which in turn leads to an inefficient electrostatic interaction with anionic siRNA and the consequent destabilization of the PIC (i.e., PGlu [DET/hydrazide]) in pyruvate-enriched environments, such as the cytoplasm, thus achieving effective siRNA release from the PIC and its associated gene-silencing activity. The present study provides the rationale for an α-oxohydrazone-formation-based smart design of pyruvate-responsive materials in the cell.
Agid:
6463934