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Analysis of antibiotic resistance of Escherichia coli isolated from the Yitong River in North-east China

Author:
Yu, Yangyang, Zhu, Xiaolin, Wu, Guanlan, Wang, Chengzhi, Yuan, Xing
Source:
Frontiers of environmental science & engineering 2019 v.13 no.3 pp. 39
ISSN:
2095-2201
Subject:
Escherichia coli, antibiotic resistance, breeding sites, coliform bacteria, fluoroquinolones, high performance liquid chromatography, livestock, livestock breeding, plate count, pollution, risk, rivers, seasonal variation, spring, sulfonamides, summer, tetracyclines, trimethoprim, China
Abstract:
The Yitong River is one of the largest secondary tributaries of the Songhua River. The area where the Yitong River flows is densely populated and contains the livestock and poultry breeding areas of northeast China. These areas introduce a high risk of antibiotic contamination. In this study, the concentrations of four types of typical antibiotics including quinolones, tetracyclines, sulfonamides, and trimethoprim were determined by solid phase extraction-high performance liquid chromatography. The antibiotic resistance of Escherichia coli caused by antibiotic pollution was investigated. The concentration of total coliforms in the Yitong River was detected by the plate counting method. The antibiotic resistance of E. coli to quinolones, tetracyclines, sulfonamides, and trimethoprim was analyzed by the Kirby-Bauer method. The results showed that the concentration of total coliforms in the summer was higher than that in the spring. There was a seasonal difference in the resistance rate of E. coli to antibiotics except trimethoprim. The antibiotic resistance to fluoroquinolones was relatively low. The resistance rate to tetracyclines was higher during the summer. Moreover, resistance to several antibiotics was observed in all sections. This study provides basic data for research on pollution characteristics and prevention of antibiotic exposure in rivers.
Agid:
6464542