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Detection of Phenylketonuria Markers Using a ZIF-67 Encapsulated PtPd Alloy Nanoparticle (PtPd@ZIF-67)-Based Disposable Electrochemical Microsensor

Xu, Xinyue, Ji, Dongqing, Zhang, Yuan, Gao, Xinghua, Xu, Pengcheng, Li, Xinxin, Liu, Chung-Chiun, Wen, Weijia
ACS applied materials & interfaces 2019 v.11 no.23 pp. 20734-20742
Fourier transform infrared spectroscopy, acylation, alloy nanoparticles, amino acid derivatives, amino acid metabolism, behavior disorders, behavior problems, congenital abnormalities, crystals, electrochemistry, electron transfer, encapsulation, imidazole, metabolites, nanocomposites, phenylacetic acid, phenylketonuria, phenylpyruvic acid, point-of-care systems, screening, seizures
Phenylketonuria (PKU) is a common disease in congenital disorder of amino acid metabolism, which can lead to intellectual disability, seizures, behavioral problems, and mental disorders. We report herein a facile method to screen for PKU by the measurements of its metabolites (markers). In this work, a disposable electrochemical microsensor modified with a ZIF (zeolitic imidazolate framework)-based nanocomposite is constructed, in which ZIF-67 crystals are encapsulated with PtPd alloy nanoparticles (NPs) forming the nanocomposite (PtPd@ZIF-67). According to electrochemical measurements, the PtPd@ZIF-67-modified microsensor shows good responses and selectivity to phenylpyruvic acid and phenylacetic acid, while almost no response toward other amino acid analogues is observed. Here, a new sensing mechanism based on the acylation reaction between the imidazole linker in ZIF-67 and carboxyl in PKU markers has been proposed and verified through the Fourier-transform infrared spectroscopy study. Moreover, the encapsulated PtPd NPs elevate the electron transfer capability of the PtPd@ZIF-67-modified microsensor and further improve the electrochemical sensing performance. Finally, we demonstrate that the developed PtPd@ZIF-67-modified microsensor has the possibility to sensing of PKU markers with high response and good specificity and may be extended to exploit the point-of-care rapid PKU screening.