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DNAJC2 is required for mouse early embryonic development

Helary, Louise, Castille, Johan, Passet, Bruno, Vaiman, Anne, Beauvallet, Christian, Jaffrezic, Florence, Charles, Mathieu, Tamzini, Mayssa, Baraige, Fabienne, Letheule, Martine, Laubier, Johann, Moazami-Goudarzi, Katayoun, Vilotte, Jean-Luc, Blanquet, Véronique, Duchesne, Amandine
Biochemical and biophysical research communications 2019 v.516 no.1 pp. 258-263
CRISPR-Cas systems, cell differentiation, chromatin, death, embryo (animal), embryogenesis, genes, messenger RNA, mice, neoplasms, small interfering RNA, stem cells
DNAJC2 protein, also known as ZRF1 or MPP11, acts both as chaperone and as chromatin regulator. It is involved in stem cell differentiation and its expression is associated with various cancer malignancies. However, the role of Dnajc2 gene during mouse embryogenesis has not been assessed so far. To this aim, we invalidated Dnajc2 gene in FVB/Nj mice using the CrispR/Cas9 approach. We showed that this invalidation leads to the early post-implantation lethality of the nullizygous embryos. Furthermore, using siRNAs against Dnajc2 in mouse 1-cell embryos, we showed that maternal Dnajc2 mRNAs may allow for the early preimplantation development of these embryos. Altogether, these data demonstrate for the first time the requirement of DNAJC2 for early mouse embryogenesis.