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Upregulated TTYH2 expression is critical for the invasion and migration of U2OS human osteosarcoma cell lines

Moon, Dong Kyu, Bae, Yeon Ju, Jeong, Geuk-Rae, Cho, Chang-Hoon, Hwang, Sun Chul
Biochemical and biophysical research communications 2019 v.516 no.2 pp. 521-525
biomarkers, gene silencing, human cell lines, humans, ion channels, osteosarcoma, small interfering RNA, therapeutics, transcription factors
Ion channels have recently emerged as stable biomarkers and anticancer targets particularly when the applications of the currently available therapeutic regimens are limited, as in case of osteosarcoma, a malignant bone tumor. Here, we evaluated the expression of TTYH2, a presumably calcium-activated chloride channel, in a human osteosarcoma cell line U2OS. We used small-interfering RNA (siRNA)-mediated gene silencing to demonstrate the downregulation in the expression of TTYH2 that resulted in the decrease in the invasion and migration, but not proliferation, of U2OS cells. The expression levels of Slug and ZEB1, the transcription factors involved in epithelial-mesenchymal transition, significantly reduced after TTYH2 silencing. Based on these results, we suggest that TTYH2 may serve as a novel target for the treatment of osteosarcoma.