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Structure elucidation and gene cluster characterization of the O-antigen of Yersinia kristensenii С-134

Author:
Sizova, Olga V., Shashkov, Alexander S., Toukach, Philip V., Knirel, Yuriy A., Shaikhutdinova, Rima Z., Ivanov, Sergei A., Kislichkina, Angelina A., Dentovskaya, Svetlana V.
Source:
Carbohydrate research 2019 v.481 pp. 9-15
ISSN:
0008-6215
Subject:
O-antigens, Yersinia kristensenii, dephosphorylation, glycerol, multigene family, nuclear magnetic resonance spectroscopy
Abstract:
Mild acid degradation of the lipopolysaccharide of Yersinia kristensenii C-134 afforded a glycerol teichoic acid-like O-polysaccharide, which was studied by sugar analysis, O-deacetylation and dephosphorylation along with 1D and 2D NMR spectroscopy. The following structure of the O-polysaccharide was established:This structure is related to those of other Y. kristensenii O-polysaccharides studied earlier. The O-antigen gene cluster of Y. kristensenii С-134 was analyzed and found to be consistent with the O-polysaccharide structure established.
Agid:
6465281