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Investigating cellular location of aquaporins in the bovine kidney. A new view on renal physiology in cattle

Michałek, Katarzyna, Grabowska, Marta
Research in veterinary science 2019 v.125 pp. 162-169
Western blotting, acid-base balance, aquaporins, body fluids, cattle, cattle diseases, epithelial cells, excretion, homeostasis, humans, immunohistochemistry, kidney diseases, kidneys, laboratory animals, veterinary medicine
To date, 13 aquaporin isoforms (AQPs) have been discovered in mammals, of which as many as 9 are located in epithelial cells lining the individual sections of the nephron and collecting tubules. Detailed analysis of the location and expression of AQPs in the kidneys of laboratory animals and humans allowed to define the key role of these proteins in renal excretion of water and other small molecules. Unfortunately, despite the significant advances in knowledge in this area, still little is known about this subject in livestock, including cattle. Therefore, the aim of the study was to determine the expression and AQPs location in the nephron segment in the bovine kidney by immunohistochemistry and Western blot. The distribution of a total of 8 aquaporins was determined as a result of the conducted experiments. The results obtained in the present study clearly indicate that aquaporins in cattle are involved in the renal regulation of water excretion and maintenance of proper acid-base balance. Undoubtedly, changes in the distribution and expression of AQPs in bovine kidneys may be the cause of water balance disorders and disruption of the normal body fluid composition. Kidney diseases in cattle are poorly described in veterinary medicine. Knowledge of cellular location and expression of all AQPs in the bovine kidney under normal physiological condition allows a deeper understanding of the renal regulation of body homeostasis. It creates new perspective for diagnosis and pharmacotherapy in cattle in the future.