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Ultrasensitive detection of long non-coding RNAs based on duplex-specific nuclease-actuated cyclic enzymatic repairing-mediated signal amplification
- ZhangThese authors contributed equally to this work., Yan, Wang, Xin-yan, Su, Xianwei, Zhang, Chun-yang
- Chemical communications 2019 v.55 no.48 pp. 6827-6830
- chemical compounds, chemical reactions, detection limit, fluorescence, human diseases, neoplasm cells, non-coding RNA
- We develop a new fluorescent method for sensitive detection of long noncoding RNAs (lncRNAs) on the basis of duplex-specific nuclease-actuated cyclic enzymatic repairing-mediated signal amplification. This method exhibits high sensitivity with a detection limit of 0.081 fM, and it can accurately detect the endogenous lncRNA HOTAIR in cancer cells, providing a new approach to study the physiological function of lncRNAs in human diseases.