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cAMP-modulated biomimetic ionic nanochannels based on a smart polymer

Author:
Chen, Zhixiang, Sun, Taolei, Qing, Guangyan
Source:
Journal of materials chemistry B 2019 v.7 no.23 pp. 3710-3715
ISSN:
2050-7518
Subject:
adsorption, aluminum oxide, biomimetics, cell membranes, composite polymers, cyclic AMP, hydrogen bonding, ions, proteins
Abstract:
The flow of ions across the cell membrane is essential to many life processes. Cyclic 3′,5′-adenosine monophosphate (cAMP) binds to and regulates the function of cyclic nucleotide-gating channel proteins, which directly mediate the Na⁺/Ca²⁺ transmembrane behaviours. This inspires us to develop a biomimetic cAMP-modulated ionic nanochannel based on a cAMP-responsive polymer design. Driven by specific hydrogen bonding interactions with cAMP, an arginine-based smart copolymer film displays remarkable adsorption toward cAMP and can clearly discriminate cAMP from other nucleotides, accompanied with reversible conformational transition of the polymer chain from a contracted state to a swollen one. Benefiting from these features, dynamic gating behaviours of the nanochannels located on the copolymer-modified anodic aluminum oxide (AAO) membrane could be precisely manipulated by cAMP. Sensitive responsiveness (10 pmol L⁻¹) and high specificity toward cAMP, high controllability and satisfactory reversibility reveal the great potential of biomolecule responsive polymers in biomimetic nanochannels and nanodevices.
Agid:
6467847