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In Vitro Multiple Shoot Induction and Plant Regeneration from Male Ephedra foliata: A Potential Medicinal Gymnosperm
- Joshi, Akshay, Deokule, Subhash
- National Academy science letters 2019 v.42 no.3 pp. 271-276
- Ephedra, alkaloids, explants, indole acetic acid, kinetin, males, rooting, shoots, therapeutics
- Ephedra foliata Boiss. Ex. C.A. Mey, known as soma plant according to ancient Indian Ayurvedic system. It is known for its potential to cure many diseases. Review of literature revealed that E. foliata contains traces of alkaloids responsible for its therapeutic activity. In present study effect of different concentrations of plant growth regulator on nodal sector explants was checked for multiple shoot production. Maximum numbers of shoots were observed on Kinetin (2.5 mg/l) whereas, Kinetin (2.5 mg/l) with Indole acetic acid (2.5 mg/l) induced 9.04 ± 0.8 shoots in 22.46 ± 0.57 days and percentage of explants that showed response was more than any other combination. Out of the two plant growth regulators used for root induction from in vitro grown shoots 1-napthaleneacetic acid showed better response than indole-3-acetic acid.