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In Vitro Antagonism of Trichoderma Isolates Against Curvularia andropogonis Causing Leaf Blight of Java Citronella

Pandey, Shailesh, Kumar, Rajesh, Giri, Krishna
National Academy science letters 2019 v.42 no.3 pp. 259-263
Curvularia, Cymbopogon winterianus, Trichoderma asperellum, Trichoderma virens, analysis of variance, antagonism, culture filtrates, forest soils, internal transcribed spacers, leaf blight, mycelium, pathogens, ribosomal DNA, sequence analysis, India
Leaf blight is the most devastating disease of Java Citronella (Cymbopogon winterianus) in North east India. The pathogen was isolated and identified as Curvularia andropogonis. A total of 6 Trichodema isolates were recovered from forest soil and tested against the target pathogen. Analysis of variance (ANOVA) revealed significant differences among Trichoderma isolates in checking the mycelial growth of C. andropogonis in dual culture, inverted plate and culture filtrate assay (Pā€‰<ā€‰0.05). Two potential Trichoderma isolates were identified as T. asperellum and T. virens using sequence analysis of internal transcribed spacer region of the ribosomal DNA. Both the species were found more effective and significantly inhibited the growth of the test pathogen in vitro. Our results suggest appealing application possibilities of Trichoderma isolates in the biological management of leaf blight disease of Java Citronella.