U.S. flag

An official website of the United States government

Dot gov

Official websites use .gov
A .gov website belongs to an official government organization in the United States.


Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Main content area

Neomycin: An Effective Inhibitor of Jasmonate-Induced Reactions in Plants

Jyothilakshmi Vadassery, Daniel J. Ballhorn, Steven R. Fleming, Christian Mazars, Shree P. Pandey, Axel Schmidt, Meredith C. Schuman, Kai-Wun Yeh, Ayufu Yilamujiang, Axel Mithöfer
Journal of plant growth regulation 2019 v.38 no.2 pp. 713-722
Ipomoea batatas, Nepenthes alata, Nicotiana attenuata, Nicotiana tabacum, Phaseolus lunatus, Picea abies, Populus trichocarpa, Triticum aestivum, aspartic proteinases, calcium, cell culture, enzyme activity, gene expression, gene induction, jasmonic acid, mutants, nectar secretion, nectaries, neomycin, plant hormones, secondary metabolites, signal transduction, sporamin
Jasmonates are important phytohormones involved in both plant developmental processes as well as defense reactions. Many JA-mediated plant defense responses have been studied in model plants using mutants of the jasmonate signaling pathway. However, in plant species where JA-signaling mutants are not accessible, the availability of a tool targeting JA signaling is crucial to investigate jasmonate-dependent processes. Neomycin is a poly-cationic aminoglycoside antibiotic that blocks the release of Ca²⁺ from internal stores. We examined the inhibitory activities of neomycin on different jasmonate-inducible responses in eight different plant species: Intracellular calcium measurements in Nicotiana tabacum cell culture, Sporamin gene induction in Ipomoea batatas, PDF2.2 gene expression in Triticum aestivum, Nepenthesin protease activity measurement in Nepenthes alata, extrafloral nectar production in Phaseolus lunatus, nectary formation in Populus trichocarpa, terpene accumulation in Picea abies, and secondary metabolite generation in Nicotiana attenuata. We are able to show that neomycin, an easily manageable and commercially available compound, inhibits JA-mediated responses across the plant kingdom.