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Purification and polymerisation of microbial d-lactic acid from DDGS hydrolysates fermentation

Zaini, Nurul Aqilah Mohd, Chatzifragkou, Afroditi, Tverezovskiy, Viacheslav, Charalampopoulos, Dimitris
Biochemical engineering journal 2019 v.150 pp. 107265
acidification, activated carbon, adsorption, anion exchange, anion exchange resins, binding capacity, condensation reactions, culture media, fermentation, hydrolysates, lactic acid, models, molecular weight, polymerization, polymers, purification methods
A multi-step process was developed for microbial d-lactic acid purification, followed by poly-d-lactic acid (PDLA) synthesis via azeotropic polycondensation process. Several anion exchange resins were screened for their binding capacity using model lactic acid solutions. Amberlite® IRA67 (weak base anion exchange resin) showed the highest lactic acid adsorption, with maximum adsorption capacity, qmax, of 136.11 mg lactic acid / g of resin, and was further selected to purify D-lactic acid from DDGS hydrolysates through a three-step process; (1) treatment with 7% w/v activated carbon, (2) acidification of fermentation broth (Amberlite® IRA120) and (3) adsorption of lactic acid by anion exchange (Amberlite® IRA67). At the end of the purification process, 80.4% (w/w) D-lactic acid was recovered with 91.8% (w/w) purity, indicating the effectiveness of the developed downstream process. Furthermore, a clear yellowish solid polymer with a molecular weight of 3010 Da was obtained, suitable for applications in biomedical and agricultural sectors.