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Development of a bacterial consortium comprising oil-degraders and diazotrophic bacteria for elimination of exogenous nitrogen requirement in bioremediation of diesel-contaminated soil

Author:
Chaudhary, Dhiraj Kumar, Bajagain, Rishikesh, Jeong, Seung-Woo, Kim, Jaisoo
Source:
World journal of microbiology & biotechnology 2019 v.35 no.7 pp. 99
ISSN:
0959-3993
Subject:
Acinetobacter, Azotobacter vinelandii, ammonium nitrate, bioremediation, nitrogen, nitrogen fixation, nitrogen-fixing bacteria, polluted soils, total nitrogen
Abstract:
The purpose of this study was to develop an effective bacterial consortium and determine their ability to overcome nitrogen limitation for the enhanced remediation of diesel-contaminated soils. Towards this, various bacterial consortia were constructed using oil-degrading and nitrogen-fixing microbes. The diesel removal efficiency of various developed consortia was evaluated by delivering the bacterial consortia to the diesel-contaminated soils. The consortium Acinetobacter sp. K-6 + Rhodococcus sp. Y2-2 + NH₄NO₃ resulted in the highest removal (85.3%) of diesel from the contaminated soil. The consortium containing two different oil-degrading microbes (K-6 + Y2-2) and one nitrogen-fixing microbe Azotobacter vinelandii KCTC 2426 removed 83.1% of the diesel from the soil after 40 days of treatment. The total nitrogen content analysis revealed higher amounts of nitrogen in soil treated with the nitrogen-fixing microbe when compared with that of the soil supplemented with exogenous inorganic nitrogen. The findings in this present study reveal that the consortium containing the nitrogen-fixing microbe degraded similar amounts of diesel to that degraded by the consortium supplemented with exogenous inorganic nitrogen. This suggests that the developed consortium K-6 + Y2-2 + KCTC 2426 compensated for the nitrogen limitation and eliminated the need for exogenous nitrogen in bioremediation of diesel-contaminated soils.
Agid:
6477954