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Activity-Based Probe for Screening of High-Colibactin Producers from Clinical Samples

Author:
Hirayama, Yuichiro, Tsunematsu, Yuta, Yoshikawa, Yuko, Tamafune, Ryota, Matsuzaki, Nobuo, Iwashita, Yuji, Ohnishi, Ippei, Tanioka, Fumihiko, Sato, Michio, Miyoshi, Noriyuki, Mutoh, Michihiro, Ishikawa, Hideki, Sugimura, Haruhiko, Wakabayashi, Keiji, Watanabe, Kenji
Source:
Organic letters 2019 v.21 no.12 pp. 4490-4494
ISSN:
1523-7052
Subject:
Escherichia coli, biosynthesis, carcinogenesis, chemical reactions, chemical structure, diagnostic techniques, enzymes, fluorescent dyes, organic compounds, screening
Abstract:
While high-colibactin-producing Escherichia coli is thought to be associated with colorectal oncogenesis, this study is complicated part due to an inability to isolate colibactin adequately. Here, we created fluorescent probes activated by ClbP, the colibactin-maturing peptidase, to identify high-colibactin-producing strains. Our probe served as a valuable clinical diagnostic tool that allowed simple high-throughput diagnostic screening of clinical samples. Furthermore, the probe also allowed identification of high-colibactin producers that would help advance our understanding of colibactin biosynthesis.
Agid:
6478157