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Characterization of the FMN-Dependent Cysteine Decarboxylase from Thioviridamide Biosynthesis

Author:
Lu, Jingxia, Li, Jiao, Wu, Yuan, Fang, Xianyang, Zhu, Jiapeng, Wang, Huan
Source:
Organic letters 2019 v.21 no.12 pp. 4676-4679
ISSN:
1523-7052
Subject:
biosynthesis, catalytic activity, chemical reactions, crystal structure, cysteine, enzymes, multigene family, mutagenesis, peptides
Abstract:
The biosynthesis of thioviridamide-like compounds has not been elucidated. Herein, we report that TvaF from the thioviridamide biosynthetic gene cluster is an FMN-dependent cysteine decarboxylase that transforms the C-terminal cysteine of precursor peptides into a thioenol motif and exhibits high substrate flexibility. We resolved the crystal structure of TvaF bound with FMN at 2.24 Å resolution. Key residues for FMN binding and catalytic activity of TvaF have been identified and evaluated by mutagenesis studies.
Agid:
6478187