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Alkaline Protease from Nocardiopsis arvandica UTMC 1492 Isolated from Saline Soil with the Ability to Produce Bioactive Protein Hydrolysate

Mohammadipanah, Fatemeh, Ghelichkhani, Farid, Khajeh, Khosro, Hamedi, Javad
Industrial biotechnology 2018 v.14 no.1 pp. 54-60
Nocardiopsis, Salsola, ammonium sulfate, antioxidants, biotechnology, enzyme activity, feed industry, fermentation, genes, liquid chromatography, pH, peptones, protein hydrolysates, proteinases, rapeseed, rhizosphere, ribosomal RNA, sales, saline soils
Proteases constitute more than half of the industrial enzymes sales and among them, alkaline proteases are of high industrial impact. The strain identified as Nocardiopsis UTMC 1492 was isolated from the rhizosphere of Salsola sp. from saline soils. Growth at high concentration of salt and high protease activity introduced the Nocardiopsis UTMC 1492 as a valuable candidate for scale up fermentation and purification of its protease. In spite of 100% identity in 16S rRNA gene of Nocardiopsis sp. UTMC 1492 to N. arvandica, cultural and physiological differences suggest that microenvironment adaptation of a single strain can lead to the significant change in the behavior of the microorganism. Enzyme purification was carried out by two-step Q-sepharose fast protein liquid chromatography (FPLC) with pH 11.0 and 8.0, followed by ammonium sulfate precipitation. This enzyme was ∼30 kDa. The partially purified enzyme showed optimum activity at 60°C and pH 9.0. This enzyme showed antioxidant producing peptones by hydrolyzing proteins extracted from rapeseed, which demonstrated its potential capability in food and feed industries.