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Expression analysis and characterization of dmrt2 in Chinese tongue sole (Cynoglossus semilaevis)

Author:
Zhu, Ying, Cui, Zhongkai, Yang, Yingming, Xu, Wenteng, Shao, Changwei, Fu, Xiaoqin, Li, Yangzhen, Chen, Songlin
Source:
Theriogenology 2019 v.138 pp. 1-8
ISSN:
0093-691X
Subject:
Cynoglossus semilaevis, amino acids, complementary DNA, females, gene expression, genes, germ cells, hatching, in situ hybridization, males, messenger RNA, methylation, open reading frames, peptides, quantitative polymerase chain reaction, testes, zinc
Abstract:
Dmrt2 is a member of the dmrt gene family with a conserved zinc finger-like DNA-binding motif (DM domain). In the present study, CS-dmrt2 was cloned from the gonads of Chinese tongue sole (Cynoglossus semilaevis). The full-length cDNA of CS-dmrt2 is 2834 bp in length, with a 251 bp 5′-untranslated region (UTR), a 1086 bp 3′-UTR and a 1503 bp open reading frame (ORF) that encodes a 501-amino-acid peptide. qPCR revealed that CS-dmrt2 was mainly expressed in C. semilaevis testes. In situ hybridization (ISH) showed CS-dmrt2 expression throughout early gonadal development (36 days after hatching (dah) and 86 dah), but the expression was higher in male gonads than in female gonads. CS-dmrt2 mRNA was highly expressed in male germ cells. Comparison of methylation levels between females and males demonstrated hypo-methylated levels of the CS-dmrt2 promoter in the male gonads, which is consistent with the high mRNA expression. These results suggest that the CS-dmrt2 gene may play a functional role in gonadal differentiation/development and germ cell maturation in the testis.
Agid:
6479017