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Expression analysis and characterization of dmrt2 in Chinese tongue sole (Cynoglossus semilaevis)
- Zhu, Ying, Cui, Zhongkai, Yang, Yingming, Xu, Wenteng, Shao, Changwei, Fu, Xiaoqin, Li, Yangzhen, Chen, Songlin
- Theriogenology 2019 v.138 pp. 1-8
- Cynoglossus semilaevis, amino acids, complementary DNA, females, gene expression, genes, germ cells, hatching, in situ hybridization, males, messenger RNA, methylation, open reading frames, peptides, quantitative polymerase chain reaction, testes, zinc
- Dmrt2 is a member of the dmrt gene family with a conserved zinc finger-like DNA-binding motif (DM domain). In the present study, CS-dmrt2 was cloned from the gonads of Chinese tongue sole (Cynoglossus semilaevis). The full-length cDNA of CS-dmrt2 is 2834 bp in length, with a 251 bp 5′-untranslated region (UTR), a 1086 bp 3′-UTR and a 1503 bp open reading frame (ORF) that encodes a 501-amino-acid peptide. qPCR revealed that CS-dmrt2 was mainly expressed in C. semilaevis testes. In situ hybridization (ISH) showed CS-dmrt2 expression throughout early gonadal development (36 days after hatching (dah) and 86 dah), but the expression was higher in male gonads than in female gonads. CS-dmrt2 mRNA was highly expressed in male germ cells. Comparison of methylation levels between females and males demonstrated hypo-methylated levels of the CS-dmrt2 promoter in the male gonads, which is consistent with the high mRNA expression. These results suggest that the CS-dmrt2 gene may play a functional role in gonadal differentiation/development and germ cell maturation in the testis.