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Açai (<i>Euterpe oleracea</i> Mart.) Seed Extract Induces Cell Cycle Arrest and Apoptosis in Human Lung Carcinoma Cells

Martinez, Raquel Martins, Guimarães, Deborah de Almeida Bauer, Berniz, Camila Ramos, Abreu, Joel Pimentel de, Rocha, Ana Paula Machado da, Moura, Roberto Soares de, Resende, Angela Castro, Teodoro, Anderson Junger
Foods 2018 v.7 no.11
2,2-diphenyl-1-picrylhydrazyl, Euterpe oleracea, apoptosis, cell cycle checkpoints, cell viability, cytotoxicity, flow cytometry, fruits, gallic acid, human cell lines, interphase, lung neoplasms, municipal solid waste, neoplasm cells, oxygen radical absorbance capacity, protective effect, seed extracts, seeds, toxicity testing
A&ccedil;ai fruit has been studied for its antioxidant properties, with positive feedback against many diseases, including cancer. Although a&ccedil;ai seeds are not edible, their composition has been studied in order to find new applications and reduce garbage generation. This study aimed to evaluate the cytotoxic effects and impacts on the cell cycle and apoptosis of a&ccedil;ai seed extract (ASE) on human lung carcinoma cell line (A549). Antioxidant activity of a&ccedil;ai seed extract (ASE) was measured by DPPH assay, Trolox Equivalent Antioxidant Capacity (ABTS/TEAC), Ferric Reducing Ability (FRAP) and Oxygen radical absorbance capacity (ORAC) assays. Human lung carcinoma cell viability (A549) was monitored by MTT assay method and the effects on cell cycle and apoptosis were measured by flow cytometry. The results indicate high antioxidant activity in ASE and high values of total phenolic compounds (37.08 &plusmn; 8.56 g gallic acid/100 g). The MTT assay showed a maximum decrease (72.07%) in the viability of A549 cells after 48 h treatment with ASE (200 &micro;g/mL). Flow cytometer analysis revealed that ASE increased the percentage of cells in G0/G1 phase and promoted a high increase of apoptotic cells when compared to the untreated cells. The present study suggests that ASE has a high antioxidant capacity and may have a protective effect against lung cancer.