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Bacterial community structure in rotating biological contactor treating coke wastewater in relation to medium composition

Ziembińska-Buczyńska, Aleksandra, Ciesielski, Sławomir, Żabczyński, Sebastian, Cema, Grzegorz
Environmental science and pollution research international 2019 v.26 no.19 pp. 19171-19179
Acinetobacter, Nitrobacter, Nitrosomonas, ammonia, bacteria, bacterial communities, biofilm, community structure, detection limit, erythrocytes, high-throughput nucleotide sequencing, nitrification, nitrites, physicochemical properties, pollutants, pollution control, wastewater, wastewater treatment
Biological wastewater treatment using biofilm systems is an effective way to treat difficult wastewater, such as coke wastewater. The information about the structure and the dynamics of this microbial community in biofilm, which are responsible for wastewater treatment, is relevant in the context of treatment efficacy and the biochemical potential to remove various pollutants. However, physico-chemical factors can influence the biofilm community significantly, causing performance disturbances. Therefore, we decided to examine the structure of microbial community in rotating biological contactor (RBC) biofilm during coke wastewater treatment and to investigate the possible shift in the community structure caused by the feeding medium change from synthetic to real coke wastewater. The experiment performed with high-throughput next-generation sequencing (NGS) revealed that bacteria commonly present in wastewater treatment plant (WWTP) systems, responsible for nitrite oxidizing, such as Nitrospira or Nitrobacter, were absent or below detection threshold, while Nitrosomonas, responsible for ammonia oxidizing, was detected in a relatively small number especially after shift to real coke wastewater. This research indicates that medium change could cause the change from autotrophic into heterotrophic nitrification led by Acinetobacter. Moreover, biofilm systems can be also a potential source of bacteria possessing high biochemical potential for pollutants removal but less known in WWTP systems, as well as potentially pathogenic microorganisms.