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Efficient biosynthesis of anticancer polysaccharide by a mutant Chaetomium globosum ALE20 via non-sterilized fermentation
- Wang, Zichao, Chen, Xuyang, Liu, Siyu, Zhang, Yingying, Wu, Zhangtao, Xu, Wenwen, Sun, Qi, Yang, Libo, Zhang, Huiru
- International journal of biological macromolecules 2019 v.136 pp. 1106-1111
- Chaetomium globosum, batch fermentation, biosynthesis, galactose, glucose, glucuronic acid, glycerol, mannose, molecular weight, mutants, polysaccharides, raw materials
- The sterilization process, due to its immense energy consumption, high facilities investment, and loss of raw materials by caramelization, during industrial production has drawn much attention. In this study, a methanol-resistant mutant strain, Chaetomium globosum ALE20, was obtained following 20 cycles of adaptive laboratory evolution process. The titer of anticancer polysaccharide (GCP-M) from C. globosum ALE20 reached 9.2 g/L with glycerol as sole carbon source using non-sterilized and fed-batch fermentation strategy. This titer represents a 200% increase compared with the 3.3 g/L attained with batch fermentation. The GCP-M monosaccharide was comprised of galactose, glucose, mannose and glucuronic acid, in a molar ratio of 3.83:66.37:3.26:1.95, respectively, and its weight-average molecular weight and polydispersity were 3.796 × 104 Da and 1.060, respectively. This work presents an ideal alternative and safer fermentation process without sterilization, and a useful approach for enhancing industrial production.