PubAg

Main content area

Reconstruction of the pulmonary artery by a novel biodegradable conduit engineered with perinatal stem cell-derived vascular smooth muscle cells enables physiological vascular growth in a large animal model of congenital heart disease

Author:
Ghorbel, Mohamed T., Jia, Huidong, Swim, Megan M., Iacobazzi, Dominga, Albertario, Ambra, Zebele, Carlo, Holopherne-Doran, Delphine, Hollander, Anthony, Madeddu, Paolo, Caputo, Massimo
Source:
Biomaterials 2019 v.217 pp. 119284
ISSN:
0142-9612
Subject:
animal models, biodegradability, bioreactors, blood, endothelial cells, endothelium, heart abnormalities, humans, immunohistochemistry, intestines, mesenchymal stromal cells, myocytes, piglets, pulmonary artery, smooth muscle, surgery, umbilical cord
Abstract:
Lack of growth potential of available grafts represents a bottleneck in the correction of congenital heart defects. Here we used a swine small intestinal submucosa (SIS) graft functionalized with mesenchymal stem cell (MSC)-derived vascular smooth muscle cells (VSMCs), for replacement of the pulmonary artery in piglets.MSCs were expanded from human umbilical cord blood or new-born swine peripheral blood, seeded onto decellularized SIS grafts and conditioned in a bioreactor to differentiate into VSMCs. Results indicate the equivalence of generating grafts engineered with human or swine MSC-derived VSMCs. Next, we conducted a randomized, controlled study in piglets (12–15 kg), which had the left pulmonary artery reconstructed with swine VSMC-engineered or acellular conduit grafts. Piglets recovered well from surgery, with no casualty and similar growth rate in either group. After 6 months, grafted arteries had larger circumference in the cellular group (28.3 ± 2.3 vs 18.3 ± 2.1 mm, P < 0.001), but without evidence of aneurism formation. Immunohistochemistry showed engineered grafts were composed of homogeneous endothelium covered by multi-layered muscular media, whereas the acellular grafts exhibited a patchy endothelial cell layer and a thinner muscular layer.show the feasibility and efficacy of pulmonary artery reconstruction using clinically available grafts engineered with allogeneic VSMCs in growing swine.
Agid:
6483490