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Immune responses upon in ovo HVT-IBD vaccination vary between different chicken lines

Author:
Dobner, Marina, Auerbach, Monika, Mundt, Egbert, Preisinger, Rudolf, Icken, Wiebke, Rautenschlein, Silke
Source:
Developmental and comparative immunology 2019 v.100 pp. 103422
ISSN:
0145-305X
Subject:
CD4-positive T-lymphocytes, Herpesviridae, Infectious bursal disease virus, chickens, genotype, humoral immunity, immune response, interferons, live vaccines, macrophages, nitric oxide, splenocytes, turkeys, vaccination, viral proteins
Abstract:
The genotype of chickens is assumed to be associated with variable immune responses. In this study a modern, moderate performing dual-purpose chicken line (DT) was compared with a high-performing layer-type (LT) as well as a broiler-type (BT) chicken line. One group of each genotype was vaccinated in ovo with a recombinant herpesvirus of turkeys expressing the virus protein VP2 of the infectious bursal disease virus (HVT-IBD) while one group of each genotype was left HVT-IBD unvaccinated (control group). Genotype associated differences in innate and adapted immune responses between the groups were determined over five weeks post hatch. HVT-IBD vaccination significantly enhanced humoral immune responses against subsequently applied live vaccines compared to non-HVT-IBD vaccinated groups at some of the investigated time points (P < 0.05). In addition HVT-IBD vaccination had depending on the genotype a significant impact on splenic macrophage as well as bursal CD4+ T-cell numbers (P < 0.05). On the other hand, the detectable genotype influence on Interferon (IFN) γ and nitric oxide (NO) release of ex vivo stimulated spleen cells was independent of HVT-IBD vaccination. The results of our study suggest considering a genotype specific vaccination regime in the field.
Agid:
6483525