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Immune responses upon in ovo HVT-IBD vaccination vary between different chicken lines

Dobner, Marina, Auerbach, Monika, Mundt, Egbert, Preisinger, Rudolf, Icken, Wiebke, Rautenschlein, Silke
Developmental and comparative immunology 2019 v.100 pp. 103422
CD4-positive T-lymphocytes, Herpesviridae, Infectious bursal disease virus, chickens, genotype, humoral immunity, immune response, interferons, live vaccines, macrophages, nitric oxide, splenocytes, turkeys, vaccination, viral proteins
The genotype of chickens is assumed to be associated with variable immune responses. In this study a modern, moderate performing dual-purpose chicken line (DT) was compared with a high-performing layer-type (LT) as well as a broiler-type (BT) chicken line. One group of each genotype was vaccinated in ovo with a recombinant herpesvirus of turkeys expressing the virus protein VP2 of the infectious bursal disease virus (HVT-IBD) while one group of each genotype was left HVT-IBD unvaccinated (control group). Genotype associated differences in innate and adapted immune responses between the groups were determined over five weeks post hatch. HVT-IBD vaccination significantly enhanced humoral immune responses against subsequently applied live vaccines compared to non-HVT-IBD vaccinated groups at some of the investigated time points (P < 0.05). In addition HVT-IBD vaccination had depending on the genotype a significant impact on splenic macrophage as well as bursal CD4+ T-cell numbers (P < 0.05). On the other hand, the detectable genotype influence on Interferon (IFN) γ and nitric oxide (NO) release of ex vivo stimulated spleen cells was independent of HVT-IBD vaccination. The results of our study suggest considering a genotype specific vaccination regime in the field.