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Deregulation of H19 is associated with cervical carcinoma

Author:
Roychowdhury, Anirban, Samadder, Sudip, Das, Pijush, Mazumder, Dipanjana Indra, Chatterjee, Ankita, Addya, Sankar, Mondal, Ranajit, Roy, Anup, Roychoudhury, Susanta, Panda, Chinmay Kumar
Source:
Genomics 2020 v.112 no.1 pp. 961-970
ISSN:
0888-7543
Subject:
gene expression regulation, gene overexpression, methylation, microarray technology, patients, prognosis, pseudogenes, quantitative polymerase chain reaction, transcription (genetics), uterine cervical neoplasms, women
Abstract:
CACX is one of the most common cancer affecting women world-wide. Here, expression microarray analysis revealed 8 over-expressed transcribed pseudogenes (GBP1P1, HLA-DRB6, HLA-H, SLC6A10P, NAPSB, KRT16P2, PTTG3P and RNF126P1), down-regulated 7 lincRNAs (H19, MIR100HG, MEG3, DIO3OS, HOXA11-AS, CD27-AS1 and EPB41L4A-AS) and 6 snoRNAs (SNORD97, SNORD3A, SNORD3C, SNORD3D, SNORA12 and SCARNA9) as DEncGs (log2 fold-change ≥ ±1.0) in CACX. Consequently, down-regulation of lincRNA MEG3 and over-expression of pseudogenes, GBP1P1 and PTTG3P in the microarray analysis were found concordant with the real-time quantitative PCR results upon validation. Then, Ingenuity® Pathway analysis (IPA®) analysis with deregulated DEncGs identified functionally important gene, H19. Further, validation (n = 52) of expression confirmed frequent downregulation of H19 with significant association with its deletion (LOH) and promoter methylation (n = 128) in CACX. Moreover, clinicopathological analysis found Indian CACX patients (n = 26) with alterations of H19 by deletion or, promoter methylation with concomitant low expression have poor prognosis.
Agid:
6484170