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Detection of endogenous retrovirus antigens in NOD mouse pancreatic β-cells
- Tsumura, H., Miyazawa, M., Ogawa, S., Wang, J. Z., Ito, Y., Shimura, K.
- Laboratory animals 1998 v.32 no.1 pp. 86-94
- antigens, genome, islets of Langerhans, mice, oligodeoxyribonucleotides, pathogenesis, viruses
- We characterized C-type retroviruses expressed in the pancreatic β-cells of non-obese diabetic (NOD) mice by immunohistochemical techniques and by inhibiting the production of viral particles using antisense oligonucleotides. Some cells in the pancreatic islets from both NOD and diabetes-resistant NOD-related mice (NON) reacted with a monoclonal antibody directed against the envelope protein(s) of polytropic viruses. On the other hand, NOD islet cells also showed strong immunoreactivity with an anti-gag protein monoclonal antibody and another anti-envelope protein(s) monoclonal antibody that is specific for xenotropic viruses. In antisense oligodeoxynucleotide inhibition assays, a xenotropic virus-specific phosphorothionate antisense oligodeoxynucleotide significantly inhibited the occurrence of C-type virus particles in NOD mouse islet β-cells. Therefore, C-type retrovirus-like particles expressed in NOD mouse pancreatic β-cells were considered to be endogenous xenotropic virus. The expression of the xenotropic viral genome may be involved in the pathogenesis of the diabetic syndrome in NOD mice.