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Incorporation of Baccharis dracunculifolia DC (Asteraceae) leaf extract into phosphatidylcholine-cholesterol liposomes improves its anti-inflammatory effect in vivo

Author:
de Figueiredo-Rinhel, Andréa Silva Garcia, de Andrade, Micássio Fernandes, Landi-Librandi, Ana Paula, Azzolini, Ana Elisa Caleiro Seixas, Kabeya, Luciana Mariko, Bastos, Jairo Kenupp, Lucisano-Valim, Yara Maria
Source:
Natural product research 2019 v.33 no.17 pp. 2521-2525
ISSN:
1478-6427
Subject:
Baccharis dracunculifolia, aerial parts, anti-inflammatory activity, caffeic acid, cholesterol, humans, inflammation, interleukins, leaf extracts, neutrophils, phosphatidylcholines, rats, superoxide anion, traditional medicine, tumor necrosis factor-alpha
Abstract:
The aerial parts of Baccharis dracunculifolia (BdE) is used in the Brazilian folk medicine to treat inflammatory conditions. Here we examined the ability of free and liposomal BdE to modulate reactive oxygen species generation in human neutrophils in vitro and zymosan-induced acute joint inflammation in Wistar rats. We prepared biocompatible liposomes of soya phosphatidylcholine and cholesterol with low diameter, homogeneous size distribution, and neutral surface charge. Free BdE decreased joint swelling, total leucocyte and neutrophil infiltration, and the synovial levels of tumour necrosis factor-α and interleukins 6 and 1β. Incorporation of BdE into liposomes preserved its capacity to inhibit the neutrophil superoxide anion and total reactive oxygen species generation, and improved its anti-inflammatory effect in vivo by decreasing the effective BdE dose by nearly sixfold. The same liposome type lowered the effective dose of caffeic acid by nearly sixteenfold. Therefore, incorporation of BdE into phosphatidylcholine-cholesterol liposomes improves its anti-inflammatory effect.
Agid:
6489016