Jump to Main Content
Bacteria-Instructed Click Chemistry between Functionalized Gold Nanoparticles for Point-of-Care Microbial Detection
- Mou, Xiao-Zhou, Chen, Xiao-Yi, Wang, Jianhao, Zhang, Zhaotian, Yang, Yanmei, Shou, Zhang-Xuan, Tu, Yue-Xing, Du, Xuancheng, Wu, Chun, Zhao, Yuan, Qiu, Lin, Jiang, Pengju, Chen, Chunying, Huang, Dong-Sheng, Li, Yong-Qiang
- ACS applied materials & interfaces 2019 v.11 no.26 pp. 23093-23101
- Escherichia coli, alkynes, azides, bacterial infections, blood sampling, catalysts, chemistry, color, colorimetry, copper, early diagnosis, magnetism, microbial detection, mobile telephones, nanogold, nanoparticles, point-of-care systems, public health, sepsis (infection), virulent strains
- Bacterial infections pose mounting public health concerns and cause an enormous medical and financial burden today. Rapid and sensitive detection of pathogenic bacteria at the point of care (POC) remains a paramount challenge. Here, we report a novel concept of bacteria-instructed click chemistry and employ it for POC microbial sensing. In this concept of bacteria-instructed click chemistry, we demonstrate for the first time that pathogenic bacteria can capture and reduce exogenous Cu²⁺ to Cu⁺ by leveraging their unique metabolic processes. The produced Cu⁺ subsequently acts as a catalyst to trigger the click reaction between gold nanoparticles (AuNPs) modified with azide and alkyne functional molecules, resulting in the aggregation of nanoparticles with a color change of the solution from red to blue. In this process, signal amplification from click chemistry is complied with the aggregation of functionalized AuNPs, thus presenting a robust colorimetric strategy for sensitive POC sensing of pathogenic bacteria. Notably, this colorimetric strategy is easily integrated in a smartphone app as a portable platform to achieve one-click detection in a mobile way. Moreover, with the help of the magnetic preseparation process, this smartphone app-assisted platform enables rapid (within 1 h) detection of Escherichia coli with high sensitivity (40 colony-forming units/mL) in the complex artificial sepsis blood samples, showing great potential for clinical early diagnosis of bacterial infections.