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Molecular Cloning and Expression Analysis of ScTUA Gene in Sugarcane

Zhang, Bao-Qing, Shao, Min, Liang, Yong-Jian, Huang, Xing, Song, Xiu-Peng, Chen, Hu, Yang, Li-Tao, Li, Yang-Rui
Sugar tech 2019 v.21 no.4 pp. 578-585
amino acid sequences, amino acids, bioinformatics, cold stress, cold tolerance, complementary DNA, genes, leaves, molecular cloning, open reading frames, polypeptides, quantitative polymerase chain reaction, reverse transcriptase polymerase chain reaction, sugarcane, tubulin
The aim of this study was to clone the full-length cDNA of sugarcane α-tubulin gene (ScTUA), investigate its sequence characteristics and analyze its expression in different resistant sugarcane varieties under cold stress. The ScTUA gene cDNA sequence was cloned from sugarcane leaf using RT-PCR techniques. The bioinformatics methods were used to analyze the putative amino acid sequence, and real-time PCR method was used to study the expression of ScTUA gene in different resistant sugarcane varieties under cold stress. The full-length cDNA of ScTUA (GenBank accession number: JQ230105) in sugarcane was cloned. The sequence included an open reading frame of 1353 bp, encoding a polypeptide of 450 amino acids. Homology analysis showed that the deduced ScTUA protein was highly homologous to other ScTUA proteins from different species. Under cold stress, real-time PCR results showed that the expression tendency of ScTUA gene in two sugarcane varieties was the same, but the relative expression level was clearly higher in cold-resistant variety GT28 than in cold-sensitive variety YL6. The gene in sugarcane had responses to cold stress, but the functions of the gene were different, and the defense pathways involved were also different in different sugarcane varieties under cold stress, which provide an important molecular basis to strengthen sugarcane resistance to cold stress and other abiotic stress.