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Online clean-up setup for the determination of non-fluorescent acidic pharmaceutical drugs in complex biological samples

Author:
Muhammad, Nadeem, Guo, Dandan, Zhang, Yun, Intisar, Azeem, Subhani, Qamar, Qadir, Muhammad Abdul
Source:
Journal of chromatography 2019
ISSN:
1570-0232
Subject:
blood serum, cost effectiveness, detection limit, drugs, fluorescence, fluorometry, ion exchange chromatography, solvents
Abstract:
Analysis of acidic pharmaceuticals in complex biological samples is a challenging and formidable task due to the existence of interfering constituents within the sample matrices. Therefore, in order to avoid analytical column clogging and suppression/enhancement of signals of the analyte of interest, herein a simple, cost-effective and quick online ion chromatography based clean-up setup was introduced. This system was further coupled with a cost-effective homemade photochemically induced fluorimetric (PIF) setup for direct online conversion of non-fluorescent acidic pharmaceutical drugs into their respective fluorescent species. This advantageous system was favorably applied for the determination of four non-fluorescent acidic compounds in two complex biological samples (human serum and oral fluid) with minimum labor and organic solvent consumption. At optimized conditions, the developed method has shown good sensitivity, selectivity, satisfactory recoveries (88.68–102.14%) and low limits of detection (0.35–8.10 μg/L) with minimum or zero matrix effect.
Agid:
6496067