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A rapid method to screen wild Solanum for resistance to early blight

Wolters, Pieter J., de Vos, Lennard, Bijsterbosch, Gerard, Woudenberg, Joyce H. C., Visser, Richard G. F., van der Linden, Gerard, Vleeshouwers, Vivianne G. A. A.
European journal of plant pathology 2019 v.154 no.1 pp. 109-114
Alternaria, Solanum, blight, crop yield, crossing, disease control, environmental factors, fungi, fungicides, genotype, germplasm, pesticide application, potatoes, rapid methods, screening, tomatoes
Early blight of potato and tomato is caused by Alternaria fungi and negatively impacts crop yields. Environmental factors and plant maturity influence disease development, which is usually kept under control by fungicide applications. Wild tuber-bearing Solanum section Petota species are a promising source of resistance to early blight that could be used to control the disease, for example by crossbreeding or modern breeding approaches. An efficient screening method is a first prerequisite for the identification of resistant genotypes in wild Solanum germplasm. Here, we describe a protocol that can be used to rapidly screen for resistance to early blight in wild Solanum collections. This protocol provides a good starting point for the identification of resistant genotypes and is a step towards breeding for resistance to early blight using wild Solanum species.