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Cloning, expression profiling, and effects of fasting status on neuropeptide Y in Schizothorax davidi

Author:
Deng, Xingxing, Lei, Luo, Yuan, Dengyue, Zheng, Zonglin, Zhu, Chengke, Luo, Hui, Ye, Hua, Li, Dongmei, Wang, Jian, Li, Baohai, Lv, Guangjun, Zhou, Chaowei
Source:
Journal of food biochemistry 2019 v.43 no.7 pp. e12892
ISSN:
0145-8884
Subject:
Schizothorax, amino acids, appetite, breeding, cold-water fish, complementary DNA, economic valuation, fasting, food intake, genes, hypothalamus, messenger RNA, neuropeptide Y, nucleotide sequences, polypeptides, refeeding, tissues, China
Abstract:
To better comprehend the mechanism that neuropeptide Y (npy) regulates feeding in Schizothorax davidi, we cloned and identified the full‐length cDNA sequence of the npy gene in this species using RACE technology. Subsequently, we explored the npy mRNA distribution in 18 tissues and investigated the expression of npy mRNA at postprandial and fasting stages. We found that the npy full‐length cDNA sequence is 803 bp. Moreover, npy mRNAs extensively expressed in all detected tissues, with the highest expression in hypothalamus. In postprandial study, the expression of npy mRNA in the hypothalamus was significantly decreased after eating (p < 0.01). In addition, the expression of the npy gene was significantly increased on the fifth day after fasting (p < 0.05). However, after refeeding, the expression of the npy gene was decreased significantly on days 9, 11, and 14 (p < 0.01). Our research suggest that npy may have an orexigenic role in S. davidi. PRACTICAL APPLICATIONS: S. davidi, a coldwater fish native to China, has high economic value, and it has gained great popularity. To date, there is still no large‐scale breeding of S. davidi in China. How to strengthen the production performance of S. davidi is a hot research area. Neuropeptide Y (NPY), a 36‐amino‐acid single‐chain polypeptide, is one of the main appetite regulation factors. However, to date, no studies have reported on the biological function of npy in the feeding of S. davidi. In our study, we revealed that the trend of hypothalamic npy expression during the postprandial and fasting stages. The results suggested that npy might be an appetite‐promoting factor in this species. Overall, we provide the theoretical basis for how to strengthen the production performance of S. davidi through appetite regulation.
Agid:
6498120