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Effect of Green Tea Extract on Systemic Metabolic Homeostasis in Diet-Induced Obese Mice Determined via RNA-Seq Transcriptome Profiles

Author:
Choi, Ji-Young, Kim, Ye Jin, Ryu, Ri, Cho, Su-Jung, Kwon, Eun-Young, Choi, Myung-Sook
Source:
Nutrients 2016 v.8 no.10
ISSN:
2072-6643
Subject:
AMP-activated protein kinase, adiposity, amino acid metabolism, animal disease models, body weight changes, branched chain amino acids, comorbidity, diet, energy expenditure, epididymis, fatty liver, glucose, green tea, health effects assessments, homeostasis, hyperlipidemia, insulin resistance, lipids, mice, obesity, sequence analysis, transcription (genetics), transcriptome, white adipose tissue
Abstract:
Green tea (GT) has various health effects, including anti-obesity properties. However, the multiple molecular mechanisms of the effects have not been fully determined. The aim of this study was to elucidate the anti-obesity effects of GT via the analysis of its metabolic and transcriptional responses based on RNA-seq profiles. C57BL/6J mice were fed a normal, high-fat (60% energy as fat), or high-fat + 0.25% (w/w) GT diet for 12 weeks. The GT extract ameliorated obesity, hepatic steatosis, dyslipidemia, and insulin resistance in diet-induced obesity (DIO) mice. GT supplementation resulted in body weight gain reduction than mice fed high-fat through enhanced energy expenditure, and reduced adiposity. The transcriptome profiles of epididymal white adipose tissue (eWAT) suggested that GT augments transcriptional responses to the degradation of branched chain amino acids (BCAAs), as well as AMP-activated protein kinase (AMPK) signaling, which suggests enhanced energy homeostasis. Our findings provide some significant insights into the effects of GT for the prevention of obesity and its comorbidities. We demonstrated that the GT extract contributed to the regulation of systemic metabolic homeostasis via transcriptional responses to not only lipid and glucose metabolism, but also amino acid metabolism via BCAA degradation in the adipose tissue of DIO mice.
Agid:
6500689