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The Anti-Inflammatory Effect of Spray-Dried Plasma Is Mediated by a Reduction in Mucosal Lymphocyte Activation and Infiltration in a Mouse Model of Intestinal Inflammation

Author:
Pérez-Bosque, Anna, Miró, Lluïsa, Amat, Concepció, Polo, Javier, Moretó, Miquel
Source:
Nutrients 2016 v.8 no.10
ISSN:
2072-6643
Subject:
Staphylococcus aureus, adhesion, animal models, anti-inflammatory activity, cattle, dietary supplements, digestive system diseases, enterotoxins, epithelium, inflammation, intercellular adhesion molecule-1, interleukin-10, intestines, lymph nodes, lymphocyte proliferation, lymphocytes, males, mice, milk proteins, patients, phosphorylation, spray drying, swine, transcription factor NF-kappa B, transforming growth factor beta, weaning
Abstract:
Spray-dried preparations from porcine and bovine plasma can alleviate mucosal inflammation in experimental models and improve symptoms in patients with enteropathy. In rodents, dietary supplementation with porcine spray-dried plasma (SDP) attenuates intestinal inflammation and improves the epithelial barrier function during intestinal inflammation induced by Staphylococcus aureus enterotoxin B (SEB). The aim of this study was to discern the molecular mechanisms involved in the anti-inflammatory effects of SDP. Male C57BL/6 mice were fed with 8% SDP or control diet (based on milk proteins) for two weeks, from weaning until day 33. On day 32, the mice were given a SEB dose (i.p., 25 µg/mouse) or vehicle. SEB administration increased cell recruitment to mesenteric lymph nodes and the percentage of activated Th lymphocytes and SDP prevented these effects). SDP supplementation increased the expression of interleukin 10 (IL-10) or transforming growth factor- β (TGF-β) compared to the SEB group. The SEB challenge increased six-fold the expression of mucosal addressin cell adhesion molecule 1 (MAdCAM-1) and intercellular adhesion molecule 1 (ICAM-1); and these effects were attenuated by SDP supplementation. SEB also augmented NF-κB phosphorylation, an effect that was prevented by dietary SDP. Our results indicate that the anti-inflammatory effects of SDP involve the regulation of transcription factors and adhesion molecules that reduce intestinal cell infiltration and the degree of the inflammatory response.
Agid:
6500707