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Antiproliferative Activity of Extracts of Campomanesia adamantium (Cambess.) O. Berg and Isolated Compound Dimethylchalcone Against B16-F10 Murine Melanoma

Magalli C.B. Lima e Silva, Danielle Bogo, Caroline A.F. Alexandrino, Renata T. Perdomo, Patrícia de O. Figueiredo, Pamela R. do Prado, Fernanda R. Garcez, Monica C.T. Kadri, Thalita V.N. Ximenes, Rita de Cassia A. Guimarães, Ulana C. Sarmento, Maria Lígia R. Macedo
Journal of medicinal food 2018 v.21 no.10 pp. 1024-1034
Campomanesia, antineoplastic activity, antineoplastic agents, apoptosis, bioactive compounds, caspase-3, cerrado, coculture, diodes, fruit pulp, fruits, high performance liquid chromatography, indigenous species, ionization, macrophages, melanoma, methylene chloride, mice, nitric oxide, phenolic compounds, spectrometers
Campomanesia adamantium, a native species of the Brazilian Cerrado, is characterized as a natural source of phenolic compounds and has known potential anticancer activities. This study aimed to evaluate the chemical profile of dichloromethane extracts of pulp (DEGPU) and peel (DEGPE) from the fruits of C. adamantium and to identify compounds with antiproliferative effects in vitro against melanoma cells by sulforhodamine B (SRB) assay, apoptosis induction assay, caspase-3 activation assay, nitric oxide (NO) release in coculture of B16-F10 cells and murine peritoneal macrophages. The chemical profiles of DEGPU and DEGPE were analyzed by high performance liquid chromatography coupled to diode array detector and mass spectrometer using the electrospray ionization interface (HPLC-DAD-ESI-MS/MS). Thirteen compounds were identified in both extracts and the chromatographic study of the most active extract in SRB assay DEGPU (GI₅₀ of 16.17 μg/mL) resulted in the isolation of seven compounds. The isolated compound dimethylchalcone (DMC) had the highest antiproliferative activity against B16-F10 with a GI₅₀ of 7.11 μg/mL. DEGPU extract activated caspase-3 in 29% of cells at 25 μg/mL and caused a 50% decrease in NO release in coculture. DEGPU can be characterized as a source of bioactive compounds such as DMC, as seen from its antiproliferative effect in vitro by inducing B16-F10 cells to undergo apoptosis, essential feature in the search for new anticancer drugs.