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Dietary leucine supplementation alters energy metabolism and induces slow-to-fast transitions in longissimus dorsi muscle of weanling piglets

Author:
Fan, Qiwen, Long, Baisheng, Yan, Guokai, Wang, Zhichang, Shi, Min, Bao, Xiaoyu, Hu, Jun, Li, Xiuzhi, Chen, Changqing, Zheng, Zilong, Yan, Xianghua
Source:
The British journal of nutrition 2017 v.117 no.9 pp. 1222-1234
ISSN:
1475-2662
Subject:
beta oxidation, bioinformatics, diet, fatty acids, gene expression regulation, glycolysis, leucine, longissimus muscle, muscle protein, oxidative phosphorylation, piglets, protein synthesis, proteins, proteome, pyruvate kinase, skeletal muscle, weanlings
Abstract:
Leucine plays an important role in promoting muscle protein synthesis and muscle remodelling. However, what percentage of leucine is appropriate in creep feed and what proteome profile alterations are caused by dietary leucine in the skeletal muscle of piglets remain elusive. In this case, we applied isobaric tags for relative and absolute quantitation to analyse the proteome profile of the longissimus dorsi muscles of weanling piglets fed a normal leucine diet (NL; 1·66 % leucine) and a high-leucine diet (HL; 2·1 % leucine). We identified 157 differentially expressed proteins between these two groups. Bioinformatics analysis of these proteins exhibited the suppression of oxidative phosphorylation and fatty acid β-oxidation, as well as the activation of glycolysis, in the HL group. For further confirmation, we identified that SDHB, ATP5F1, ACADM and HADHB were significantly down-regulated (P<0·01, except ATP5F1, P<0·05), whereas the glycolytic enzyme pyruvate kinase was significantly up-regulated (P<0·05) in the HL group. We also show that enhanced muscle protein synthesis and the transition from slow-to-fast fibres are altered by leucine. Together, these results indicate that leucine may alter energy metabolism and promote slow-to-fast transitions in the skeletal muscle of weanling piglets.
Agid:
6503636