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Metabolomic analysis of quorum sensing inhibitor hordenine on Pseudomonas aeruginosa

Zhou, Jin-Wei, Muhammad, Junaid, Sun, Bing, Yang, Rui, Wadood, Abdul, Wang, Jun-Song, Jia, Ai-Qun
Applied microbiology and biotechnology 2019 v.103 no.15 pp. 6271-6285
Pseudomonas aeruginosa, amino acid metabolism, antioxidant enzymes, bacteria, cross infection, energy metabolism, gene expression regulation, genes, hordenine, magnetism, membrane permeability, metabolites, metabolomics, oxidative stress, pathogenicity, pathogens, pyocyanin, quorum sensing
Proton magnetic resonance–based metabolomics analysis was performed to determine the global metabolite changes in pathogenic bacterium Pseudomonas aeruginosa PAO1 following exposure to quorum sensing (QS) inhibitor hordenine. Pyocyanin inhibition assay confirmed that hordenine exhibited potent QS inhibitory activity. A total of 40 metabolites were assigned by PMR spectra. Hordenine treatment resulted in the destruction of QS system in P. aeruginosa PAO1 by downregulating the expressions of genes involved in QS. The synthesis of antioxidant enzymes was repressed and the oxidative stress was enhanced due to the dysfunctional QS system of P. aeruginosa PAO1. The enhanced oxidative stress induced by the dysfunctional QS system of P. aeruginosa PAO1 altered the membrane components, enhanced membrane permeability, and disturbed energy metabolism, amino acid metabolism, and nucleotide metabolism, and would ultimately attenuate the pathogenicity of P. aeruginosa PAO1. Hordenine may have promising potential for controlling nosocomial pathogens.